extracellular

vesicles.

Biomaterials

105:195–205

7. Mitchell JP et al (2008) Increased exosome

production from tumour cell cultures using

the Integra CELLine culture system. J Immu-

nol Methods 335(1–2):98–105

8. Guerreiro EM et al (2018) Efficient extracellu-

lar vesicle isolation by combining cell media

modifications,

ultrafiltration,

and

size-

exclusion chromatography. PLoS One 13(9):

e0204276

9. Hisey CL et al (2020) Towards establishing

extracellular vesicle-associated RNAs as bio-

markers for HER2+ breast cancer. F1000Res

9:1362

10. Palviainen M et al (2019) Metabolic signature

of extracellular vesicles depends on the cell

culture

conditions.

J

Extracell

Vesicles

8

(1):1596669

11. Jeppesen DK et al (2014) Quantitative proteo-

mics of fractionated membrane and lumen exo-

some proteins from isogenic metastatic and

nonmetastatic bladder cancer cells reveal differ-

ential expression of EMT factors. Proteomics

14(6):699–712

12. Griffiths SG et al (2017) Differential proteome

analysis of extracellular vesicles from breast

cancer cell lines by chaperone affinity enrich-

ment. Proteomes 5(4):25

13. Ji H et al (2014) Deep sequencing of RNA

from

three

different

extracellular

vesicle

(EV)

subtypes

released

from

the

human

LIM1863 colon cancer cell line uncovers dis-

tinct

miRNA-enrichment

signatures.

PLoS

One 9(10):e110314

14. Chen M et al (2019) Distinct shed microvesicle

and exosome microRNA signatures reveal

diagnostic markers for colorectal cancer. PLoS

One 14(1):e0210003

15. Thery C et al (2018) Minimal information for

studies

of

extracellular

vesicles

2018

(MISEV2018): a position statement of the

International Society for Extracellular Vesicles

and update of the MISEV2014 guidelines. J

Extracell Vesicles 7(1):1535750

16. Lobb R, Moller A (2017) Size exclusion chro-

matography: a simple and reliable method for

exosome purification. In: Kuo WP, Shidong J

(eds) Extracellular vesicles: methods and pro-

tocols. Humana Press, Totowa, New Jersey, pp

105–110

17. Cizmar P, Yuana Y (2017) Detection and char-

acterization of extracellular vesicles by trans-

mission

and

cryo-transmission

electron

microscopy. In: Kuo WP, Shidong J (eds)

Extracellular vesicles: methods and protocols.

Humana

Press,

Totowa,

New

Jersey,

pp

221–232

18. Kowal EJK et al (2017) Extracellular vesicle

isolation and analysis by western blotting. In:

Kuo WP, Shidong J (eds) Extracellular vesicles:

methods

and

protocols.

Humana

Press,

Totowa, New Jersey, pp 143–152

192

Anastasiia Artuyants et al.